Blum的問題，透過圖書和論文來找解法和答案更準確安心。 我們找到下列問答集和資訊懶人包

Audience-Ology: How Moviegoers Shape the Films We Love

為了解決Blum的問題，作者Goetz, Kevin 這樣論述：

Discover the fascinating and secretive process of audience testing of Hollywood movies through these firsthand stories from famous filmmakers, studio heads, and stars.Audience-ology takes you to one of the most unknown places in Hollywood--a place where famous directors are reduced to tears and m

ulti-millionaire actors to fits of rage. A place where dreams are made and fortunes are lost. From ＂the best in the business＂ (Sacha Baron Cohen), this book is the chronicle of how real people have written and rewritten America’s cinematic masterpieces by showing up, watching a rough cut of a new fi

lm, and giving their unfettered opinions so that directors and studios can salvage their blunders, or better yet, turn their movies into all-time classics. Each chapter informs an aspect or two of the test-screening process and then, through behind-the-scenes stories, illustrates how that particular

aspect was carried out. Nicknamed ＂the doctor of audience-ology,＂ Kevin Goetz shares how he helped filmmakers and movie execs confront the misses and how he recommended ways to fix the blockbusters, as well as first-hand accounts from Ron Howard, Cameron Crowe, Ed Zwick, Renny Harlin, Jason Blum, a

nd other Hollywood luminaries who brought you such films as La La Land, Chicago, Titanic, Wedding Crashers, Jaws, and Forrest Gump. Audience-ology explores one of the most important (and most underrated) steps in the filmmaking process with enough humor, drama, and surprise to entertain those with

only a spectator’s interest in film, offering us a new look at movie history.

Blum進入發燒排行的影片

An Integrated Approach For Uncovering Novel DNA Methylation Biomarkers For Non-small Cell Lung Carcinoma

為了解決Blum的問題，作者VAIBHAV KUMAR SUNKARIA 這樣論述：

Introduction - Lung cancer is one of primal and ubiquitous cause of cancer related fatalities in the world. Leading cause of these fatalities is non-small cell lung cancer (NSCLC) with a proportion of 85%. The major subtypes of NSCLC are Lung Adenocarcinoma (LUAD) and Lung Small Cell Carcinoma (LUS

C). Early-stage surgical detection and removal of tumor offers a favorable prognosis and better survival rates. However, a major portion of 75% subjects have stage III/IV at the time of diagnosis and despite advanced major developments in oncology survival rates remain poor. Carcinogens produce wide

spread DNA methylation changes within cells. These changes are characterized by globally hyper or hypo methylated regions around CpG islands, many of these changes occur early in tumorigenesis and are highly prevalent across a tumor type.Structure - This research work took advantage of publicly avai

lable methylation profiling resources and relevant comorbidities for lung cancer patients extracted from meta-analysis of scientific review and journal available at PubMed and CNKI search which were combined systematically to explore effective DNA methylation markers for NSCLC. We also tried to iden

tify common CpG loci between Caucasian, Black and Asian racial groups for identifying ubiquitous candidate genes thoroughly. Statistical analysis and GO ontology were also conducted to explore associated novel biomarkers. These novel findings could facilitate design of accurate diagnostic panel for

practical clinical relevance.Methodology - DNA methylation profiles were extracted from TCGA for 418 LUAD and 370 LUSC tissue samples from patients compared with 32 and 42 non-malignant ones respectively. Standard pipeline was conducted to discover significant differentially methylated sites as prim

ary biomarkers. Secondary biomarkers were extracted by incorporating genes associated with comorbidities from meta-analysis of research articles. Concordant candidates were utilized for NSCLC relevant biomarker candidates. Gene ontology annotations were used to calculate gene-pair distance matrix fo

r all candidate biomarkers. Clustering algorithms were utilized to categorize candidate genes into different functional groups using the gene distance matrix. There were 35 CpG loci identified by comparing TCGA training cohort with GEO testing cohort from these functional groups, and 4 gene-based pa

nel was devised after finding highly discriminatory diagnostic panel through combinatorial validation of each functional cluster.Results – To evaluate the gene panel for NSCLC, the methylation levels of SCT(Secritin), FOXD3(Forkhead Box D3), TRIM58(Tripartite Motif Containing 58) and TAC1(Tachikinin

1) were tested. Individually each gene showed significant methylation difference between LUAD and LUSC training cohort. Combined 4-gene panel AUC, sensitivity/specificity were evaluated with 0.9596, 90.43%/100% in LUAD; 0.949, 86.95%/98.21% in LUSC TCGA training cohort; 0.94, 85.92%/97.37 in GEO 66

836; 0.91,89.17%/100% in GEO 83842 smokers; 0.948, 91.67%/100% in GEO83842 non-smokers independent testing cohort. Our study validates SCT, FOXD3, TRIM58 and TAC1 based gene panel has great potential in early recognition of NSCLC undetermined lung nodules. The findings can yield universally accurate

and robust markers facilitating early diagnosis and rapid severity examination.

The Wondrous Wonders

為了解決Blum的問題，作者Jourdy, Camille 這樣論述：

Camille Jourdy grew up in Dole, in eastern France, and studied at the Beaux-Arts in Epinal and the School of Decorative Arts in Strasbourg. Her previous works include Juliette: les fantômes reviennent au printemps and Rosalie Blum which was adapted into a film. She also works as an illustrator on al

bums and games for Actes Sud, Albin Michel, and more.

甲基化CpG 序列結構與機械性質之分子動態模擬研究

為了解決Blum的問題，作者洪欣筠 這樣論述：

甲基化DNA為表觀遺傳修飾的一種，在DNA序列不改變的前提下，胞嘧啶中C5的氫原子被催化為甲基團，以微小的差異調控基因表達︒在人類啟動子中的CpG island(CGI)若被甲基化，基因表達量會隨著在CGI中的甲基化濃度越高而下降︒目前對甲基化DNA的理解是甲基化胞嘧啶不會改變雙螺旋DNA的二級結構，反而使局部CGI的磷酸根與五碳糖骨架活動能力下降，且也讓鹼基對間的堆疊結構改變。在這篇研究中，我們為了要暸解被甲基化的胞嘧啶在細節上如何改變CGI局部的DNA結構，設計七種序列為CpG的DNA，利用GROMACS 軟體進行全原子的分子動態模擬，藉著分析分子模擬軌跡檔並應用重原子彈性網路模型理解原

子間剛性的關係，我們瞭解到甲基化後的CpG DNA仍維持B型型態，也發現甲基化鹼基對與相鄰兩個鹼基對的堆疊結構改變︒甲基化胞嘧啶先影響與之相連的氮苷鍵穩定度與旋轉角度，再促使五碳糖轉變為O4’endo構型，改變的五碳糖褶皺構型延伸影響到骨架扭轉角，進而改變相鄰鹼基對的結構與分子穩定度︒藉著我們分子模擬得到的分析結果，我們為甲基化改變CGI局部DNA 結構的機制提供分子層級的看法︒